|First Appearance||Meet the Wario Bros|
|Latest Appearance||Death of Mario|
Meet the Wario Bros.
In Meet the Wario Bros., Wario creates Waluigi using Luigi's DNA and his chemistry set. He then teaches the Cute Mario Bros. a lesson, but it is a school lesson about Christopher Columbus. Then, Wario teaches Waluigi how to be evil by training him to watch stuff. They then fight the Cute Mario Bros. again, but Mario shoots him out the window.
Death of Mario
- In Summer Fun 2, Jack mentions going camping with Wario and Waluigi, but goes camping with Mario and Luigi instead.
- Waluigi is like a version of Luigi but not scared of a lot of things.